EVOM™ Manual Shortens TEER Analysis Experimentation Time
Comparing EVOM™ Manual & EVOM2

BENEFITS OF EVOM™ MANUAL

	Store data automatically and save time! Eliminates errors and reduces experimental processing time.
	Smart touch screen display of live data and a user interface to simplify experiments. It automatically logs data, eliminating the need to track data by hand.
	Automatic low, and high resistance range options let you analyze different cell or tissue types.
	Low noise design offers greater resolution and accuracy.
	It prevents data loss with automatic save and data recovery when the battery is low.

BACKGROUND & INTRODUCTION

EVOM™ based TEER measurement is considered the gold standard for the transepithelial electrical resistance (TEER) measurement to evaluate cellular confluence, barrier function, permeability, and tissue function. Currently, WPI offers a manual system called EVOM™ Manual with the SMARTouch control screen, data storage capability, and other advanced features.

Part 1: Bronchial Epithelial Cell Model

Corning 3470 (6.5 mm diameter with 0.4 μm pore polyester membrane) 24 well plate Transwells (N=12) were seeded with 16HBE14o- (Human Bronchial Epithelial Cells) at a cell seeding density of 1.5 × 105 cells/well. These cells were grown with 0.2 mL apical, and 0.5 mL basolateral media volumes. The resistance values of blank Transwells (without any cell seeding) with same media volumes as samples were subtracted from sample resistance values to obtain resistance contributed by cellular layers. TEER values were calculated by multiplying resistance with membrane area (cell growth area= 0.33 cm²).

Part 2: Caco2 Intestinal Epithelial Model

The Caco-2 cells (HTB-37) were seeded in sixteen inserts of two 24-well Transwell plates (Corning 3470; 8 seeded inserts/plate) from passage 14 at a cell seeding density of 5.89 × 104 cells/insert with 0.1 mL of apical, and 0.6 mL basolateral media volumes. The cell line was cultured for 21 days, and transepithelial electrical resistance (TEER) values were measured on days 3, 7, 14, and 21. Four (4) unseeded inserts per plate were assigned as blanks for the assay.

SUMMARY OF RESULTS

As shown with two different cellular models, the TEER measurement can be very similar when measured with EVOM2, and EVOM™ Manual. EVOM™ Manual allows the ability to store the data in the USB flash drive in Excel/CSV format, whereas in EVOM2 users need to manually write down individual displayed data on a lab notebook, and later manually enter into an Excel data sheet. Also, in EVOM™ Manual, one can easily record data using a footswitch, in EVOM2 it was not possible. With improved internal electronics of EVOM™ Manual, it does not need 20 minutes of warm up time to achieve stable readings after powering on the system. Due to all these benefits, the EVOM™ Manual can shorten the TEER measurement experimentation time 2-6 times. Since EVOM™ Manual does not require data to be manually written down by the user, it minimizes or eliminates the chances of errors associated with manual data recording. And, EVOM™ Manual can be adopted by EVOM, EVOM2, ERS, and ERS-2 users to get comparable data with the ability to complete the TEER experimentation faster, and obtain more reliable data.

For all the data and details, download the full application note!

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FOR A TRADE-IN

EVOM™ TEER family of products has special pricing options and trade-in value for discontinued units. Complete this form to discuss your options with your Account Executive.

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